Isolation:Article Title: Single-Cell Profiling of AKI in a Murine Model Reveals Novel Transcriptional Signatures, Profibrotic Phenotype, and Epithelial-to-Stromal Crosstalk
Article Snippet: .. Total RNA was isolated from the homogenized whole-kidney lysates with RNA Stat-60 extraction reagent (CS-111; Amsbio) and purified using the GeneJET RNA purification kit (KO732; Thermo Fisher Scientific). .. The cDNAwas synthesized with the iScript Reverse Transcription Supermix (1708841; Bio-Rad). qPCR was performed with TaqMan Universal PCR Master Mix (4304437; Thermo Fisher Scientific) on the Applied Biosystems QuantStudio 3 Real-Time PCR system.
Article Title: Single-cell sequencing dissects the transcriptional identity of activated fibroblasts and identifies novel persistent distal tubular injury patterns in kidney fibrosis
Article Snippet: .. Total RNA was isolated from homogenized control, UIR and UUO whole kidney lysates (n=4-6 per group) with RNA Stat-60 extraction reagent (Amsbio, CS-111) and purified using the GeneJET RNA purification kit (ThermoFisher Scientific, KO732). .. Total RNA and protein were simultaneously isolated from RPTECs using Ambion PARIS kit (AM1921, ThermoFisher Scientific). cDNA was synthesized with the iScript Reverse Transcription Supermix (Bio-Rad, 1708841). qPCR was performed with TaqMan universal PCR master mix (Thermo Fisher Scientific, 4304437) on the Applied Biosystems Quant Studio 3 system.
Article Title: Single Cell Profiling of Acute Kidney Injury Reveals Novel Transcriptional Signatures, Mixed Identities and Epithelial-to-Stromal Crosstalk
Article Snippet: .. Total RNA was isolated from the homogenized whole kidney lysates with RNA Stat-60 extraction reagent (Amsbio, CS-111) and purified using the GeneJET RNA purification kit (Thermo Fisher Scientific, KO732). .. The cDNA was synthesized with the iScript Reverse Transcription Supermix (Bio-Rad, 1708841). qPCR was performed with TaqMan universal PCR master mix (Thermo Fisher Scientific, 4304437) on the Applied Biosystems Quant Studio 3 RT PCR system.
Article Title: Single-cell transcriptomic profiling of kidney fibrosis identifies a novel specific fibroblast marker and putative disease target
Article Snippet: .. Total RNA was isolated from homogenized control, UIR and UUO whole kidney lysates (n=4-6 per group) with RNA Stat-60 extraction reagent (Amsbio, CS-111) and purified using the GeneJET RNA purification kit (ThermoFisher Scientific, KO732). .. Total RNA and protein were simultaneously isolated from RPTECs using Ambion PARIS kit (AM1921, ThermoFisher Scientific). cDNA was synthesized with the iScript Reverse Transcription Supermix (Bio-Rad, 1708841). qPCR was performed with TaqMan universal PCR master mix (Thermo Fisher Scientific, 4304437) on the Applied Biosystems Quant Studio 3 system.
Extraction:Article Title: Single-Cell Profiling of AKI in a Murine Model Reveals Novel Transcriptional Signatures, Profibrotic Phenotype, and Epithelial-to-Stromal Crosstalk
Article Snippet: .. Total RNA was isolated from the homogenized whole-kidney lysates with RNA Stat-60 extraction reagent (CS-111; Amsbio) and purified using the GeneJET RNA purification kit (KO732; Thermo Fisher Scientific). .. The cDNAwas synthesized with the iScript Reverse Transcription Supermix (1708841; Bio-Rad). qPCR was performed with TaqMan Universal PCR Master Mix (4304437; Thermo Fisher Scientific) on the Applied Biosystems QuantStudio 3 Real-Time PCR system.
Article Title: Single-cell sequencing dissects the transcriptional identity of activated fibroblasts and identifies novel persistent distal tubular injury patterns in kidney fibrosis
Article Snippet: .. Total RNA was isolated from homogenized control, UIR and UUO whole kidney lysates (n=4-6 per group) with RNA Stat-60 extraction reagent (Amsbio, CS-111) and purified using the GeneJET RNA purification kit (ThermoFisher Scientific, KO732). .. Total RNA and protein were simultaneously isolated from RPTECs using Ambion PARIS kit (AM1921, ThermoFisher Scientific). cDNA was synthesized with the iScript Reverse Transcription Supermix (Bio-Rad, 1708841). qPCR was performed with TaqMan universal PCR master mix (Thermo Fisher Scientific, 4304437) on the Applied Biosystems Quant Studio 3 system.
Article Title: Single Cell Profiling of Acute Kidney Injury Reveals Novel Transcriptional Signatures, Mixed Identities and Epithelial-to-Stromal Crosstalk
Article Snippet: .. Total RNA was isolated from the homogenized whole kidney lysates with RNA Stat-60 extraction reagent (Amsbio, CS-111) and purified using the GeneJET RNA purification kit (Thermo Fisher Scientific, KO732). .. The cDNA was synthesized with the iScript Reverse Transcription Supermix (Bio-Rad, 1708841). qPCR was performed with TaqMan universal PCR master mix (Thermo Fisher Scientific, 4304437) on the Applied Biosystems Quant Studio 3 RT PCR system.
Article Title: Single-cell transcriptomic profiling of kidney fibrosis identifies a novel specific fibroblast marker and putative disease target
Article Snippet: .. Total RNA was isolated from homogenized control, UIR and UUO whole kidney lysates (n=4-6 per group) with RNA Stat-60 extraction reagent (Amsbio, CS-111) and purified using the GeneJET RNA purification kit (ThermoFisher Scientific, KO732). .. Total RNA and protein were simultaneously isolated from RPTECs using Ambion PARIS kit (AM1921, ThermoFisher Scientific). cDNA was synthesized with the iScript Reverse Transcription Supermix (Bio-Rad, 1708841). qPCR was performed with TaqMan universal PCR master mix (Thermo Fisher Scientific, 4304437) on the Applied Biosystems Quant Studio 3 system.
Purification:Article Title: Single-Cell Profiling of AKI in a Murine Model Reveals Novel Transcriptional Signatures, Profibrotic Phenotype, and Epithelial-to-Stromal Crosstalk
Article Snippet: .. Total RNA was isolated from the homogenized whole-kidney lysates with RNA Stat-60 extraction reagent (CS-111; Amsbio) and purified using the GeneJET RNA purification kit (KO732; Thermo Fisher Scientific). .. The cDNAwas synthesized with the iScript Reverse Transcription Supermix (1708841; Bio-Rad). qPCR was performed with TaqMan Universal PCR Master Mix (4304437; Thermo Fisher Scientific) on the Applied Biosystems QuantStudio 3 Real-Time PCR system.
Article Title: Single-cell sequencing dissects the transcriptional identity of activated fibroblasts and identifies novel persistent distal tubular injury patterns in kidney fibrosis
Article Snippet: .. Total RNA was isolated from homogenized control, UIR and UUO whole kidney lysates (n=4-6 per group) with RNA Stat-60 extraction reagent (Amsbio, CS-111) and purified using the GeneJET RNA purification kit (ThermoFisher Scientific, KO732). .. Total RNA and protein were simultaneously isolated from RPTECs using Ambion PARIS kit (AM1921, ThermoFisher Scientific). cDNA was synthesized with the iScript Reverse Transcription Supermix (Bio-Rad, 1708841). qPCR was performed with TaqMan universal PCR master mix (Thermo Fisher Scientific, 4304437) on the Applied Biosystems Quant Studio 3 system.
Article Title: Single Cell Profiling of Acute Kidney Injury Reveals Novel Transcriptional Signatures, Mixed Identities and Epithelial-to-Stromal Crosstalk
Article Snippet: .. Total RNA was isolated from the homogenized whole kidney lysates with RNA Stat-60 extraction reagent (Amsbio, CS-111) and purified using the GeneJET RNA purification kit (Thermo Fisher Scientific, KO732). .. The cDNA was synthesized with the iScript Reverse Transcription Supermix (Bio-Rad, 1708841). qPCR was performed with TaqMan universal PCR master mix (Thermo Fisher Scientific, 4304437) on the Applied Biosystems Quant Studio 3 RT PCR system.
Article Title: Single-cell transcriptomic profiling of kidney fibrosis identifies a novel specific fibroblast marker and putative disease target
Article Snippet: .. Total RNA was isolated from homogenized control, UIR and UUO whole kidney lysates (n=4-6 per group) with RNA Stat-60 extraction reagent (Amsbio, CS-111) and purified using the GeneJET RNA purification kit (ThermoFisher Scientific, KO732). .. Total RNA and protein were simultaneously isolated from RPTECs using Ambion PARIS kit (AM1921, ThermoFisher Scientific). cDNA was synthesized with the iScript Reverse Transcription Supermix (Bio-Rad, 1708841). qPCR was performed with TaqMan universal PCR master mix (Thermo Fisher Scientific, 4304437) on the Applied Biosystems Quant Studio 3 system.
Control:Article Title: Single-cell sequencing dissects the transcriptional identity of activated fibroblasts and identifies novel persistent distal tubular injury patterns in kidney fibrosis
Article Snippet: .. Total RNA was isolated from homogenized control, UIR and UUO whole kidney lysates (n=4-6 per group) with RNA Stat-60 extraction reagent (Amsbio, CS-111) and purified using the GeneJET RNA purification kit (ThermoFisher Scientific, KO732). .. Total RNA and protein were simultaneously isolated from RPTECs using Ambion PARIS kit (AM1921, ThermoFisher Scientific). cDNA was synthesized with the iScript Reverse Transcription Supermix (Bio-Rad, 1708841). qPCR was performed with TaqMan universal PCR master mix (Thermo Fisher Scientific, 4304437) on the Applied Biosystems Quant Studio 3 system.
Article Title: Single-cell transcriptomic profiling of kidney fibrosis identifies a novel specific fibroblast marker and putative disease target
Article Snippet: .. Total RNA was isolated from homogenized control, UIR and UUO whole kidney lysates (n=4-6 per group) with RNA Stat-60 extraction reagent (Amsbio, CS-111) and purified using the GeneJET RNA purification kit (ThermoFisher Scientific, KO732). .. Total RNA and protein were simultaneously isolated from RPTECs using Ambion PARIS kit (AM1921, ThermoFisher Scientific). cDNA was synthesized with the iScript Reverse Transcription Supermix (Bio-Rad, 1708841). qPCR was performed with TaqMan universal PCR master mix (Thermo Fisher Scientific, 4304437) on the Applied Biosystems Quant Studio 3 system.
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